Journal: American Journal of Translational Research
Article Title: Integrin-associated CD151 is a suppressor of prostate cancer progression
doi:
Figure Lengend Snippet: Reprehensive image of FAK staining in human prostate tumors. A. TMA from the local prostate cancer patient cohort was subjected to IHC analysis with an FAK-specific antibody. a-f. FAK staining in tumors with benign feature or varying in Gleason grade or stage. Scale: 100×, 200× insert. B. MTT analysis of CD151 knockdown on tumor cell sensitivity to FAK inhibitor (VS-6063) or chemotherapeutic agent (Docetaxel). BPH Tumor cells with or without stable knockdown of CD151 were treated with indicated agents for 72 h, followed by analyses of cell viability by MTT assay and paired t-test analysis. *: p value <0.05; **: p value <0.01. C. FAK deregulation at genomic and mRNA levels and association with oncogenic drivers in the TCGA prostate cancer patient cohort (Cell, 2015). a, b. Association between mRNA expression of FAK and gene copy number. CD151 mRNA expression and Gleason grade. c-e. Plots of FAK mRNA expression and tumor Gleason grade, AR and p53.
Article Snippet: The antibodies against pY 416 -Src or pY 397 and total FAK were obtained from Cell Signaling Technology (Danver, MA) and Santa Cruz Biotechnology (Santa Cruz, CA), respectively.
Techniques: Staining, Knockdown, MTT Assay, Expressing